Name: GSM7573114
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC SINGLE CELL
Selection: cDNA
Layout: PAIRED
Construction protocol: Fresh normal mucosa and MCA tissue were washed with ice-cold PBS and minced into small pieces. Then the normal and tumor tissue pieces were digested with collagenase VIII at 0.38 mg/mL and DNase I at 0.1 mg/mL in 30% FBS for 1.0h at 37 °C. After digestion, tissue pieces were washed with PBS twice. Library was constructed according to the manufacter's instructions (single cell 3' V2 protocol 10 X genomics)