GSM7308659: BS-seq-WT-rep 2; Zea mays; Bisulfite-Seq - SRA

SRX20809083: GSM7308659: BS-seq-WT-rep 2; Zea mays; Bisulfite-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 98.7M spots, 29.6G bases, 9Gb downloads

External Id: GSM7308659_r1

Submitted by: Yan He Lab, ational Maize Improvement Center of China, China Agricultural University

Study: Natural polymorphisms in ZMET2 encoding a DNA methyltransferase modulate the number of husk layers in maize

PRJNA988518 • SRP446329 • All experiments • All runs

show Abstracthide Abstract

The goals of this study are to investigate the effect of ZMET2 on genome-wide DNA methylation and gene expression. Overall design: RNA sequencing (RNA-seq) and bisulfite sequencing(BS-seq) was performed using ear samples at the early growing stage of wild type and zmet2 mutant plants.

Sample: BS-seq-WT-rep 2

SAMN36026912 • SRS18092591 • All experiments • All runs

Organism: Zea mays

Library:

Name: GSM7308659

Instrument: Illumina NovaSeq 6000

Strategy: Bisulfite-Seq

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Construction protocol: Genomic DNA was isolated from the developing ear (<0.5 cm in length) with the traditional CTAB method. Samples were fragmented and ligated with TruSeq-methylated adapters. Bisulfite conversion was performed on 500 ng of adaptor-ligated DNA using the MethylCode bisulfite conversion kit (Zymo Research, Orange, CA, USA). Converted DNA was amplified for 14 cycles.

Runs: 1 run, 98.7M spots, 29.6G bases, 9Gb

Run	# of Spots	# of Bases	Size	Published
SRR25054801	98,716,713	29.6G	9Gb	2024-03-04

ID:: 28240405

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