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SRX20514607: GSM7427137: water inoculated spikelet in CDC Falcon 2; Triticum aestivum; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 28.5M spots, 7.1G bases, 2.2Gb downloads

External Id: GSM7427137_r1
Submitted by: Agriculture and Agri-Food Canada
Study: Dual RNA-sequencing of Fusarium Head Blight resistance in winter wheat
show Abstracthide Abstract
In this study, we used dual RNA-sequencing to profile FHB-resistant AC Emerson, FHB-moderately AC Morley, and FHB-susceptible CDC Falcon winter wheat cultivars prior to and in response to Fusarium graminearum at 7 days post inoculation. Differential expression analyses revealed distinct defense responses between resistant and susceptible wheat cultivars including increased mechanical defense through lignin biosynthesis and increased deoxynivalenol (DON) detoxification through UDP-glycosyltransferase activity in resistant cultivars. Further, differential expression analysis in F. graminearum challenging these distinct cultivars revealed changes genes involved in trichothecene mycotoxin biosynthesis. Overall design: FHB-resistant AC Emerson, FHB-moderately resistant AC Morley and FHB-susceptible CDC Falcon spikelets at 0 and 7 days post inoculation with Fusarium graminearum
Sample: water inoculated spikelet in CDC Falcon 2
SAMN35363545 • SRS17820075 • All experiments • All runs
Library:
Name: GSM7427137
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA extraction was performed from sectioned rachis node and rachilla samples using the Trizol Reagent (Ambion), according to the manufacturer's instructions RNA libraries were prepared for sequencing using standard illumina protocols
Runs: 1 run, 28.5M spots, 7.1G bases, 2.2Gb
Run# of Spots# of BasesSizePublished
SRR2473727328,453,3317.1G2.2Gb2024-01-24

ID:
27938290

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