GSM7140112: sample13_CT_MenACWY_-ve; Homo sapiens; RNA-Seq - SRA

SRX19856995: GSM7140112: sample13_CT_MenACWY_-ve; Homo sapiens; RNA-Seq
1 OXFORD_NANOPORE (PromethION) run: 82M spots, 62.8G bases, 55.3Gb downloads

External Id: GSM7140112_r1

Submitted by: Oxford Vaccine Group, Paediatrics, University of Oxford

Study: COVID-19 vaccination is associated with attenuation of inflammatory responses during breakthrough disease [long-read RNA-Seq]

PRJNA951717 • SRP430686 • All experiments • All runs

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A subset of individualsenrolledina randomised controlled trial ofChAdOx1 nCoV-19 and received either ChAdOx1 COVID-19 or control MenACWY vaccine whoexperienced COVID-19-associated symptoms were evaluated using multi-omictechnologies. Participants were selected to represent symptomatic individuals with and without COVID-19 as determined by a nucleic acid amplification test (NAAT). Overall design: Blood samples taken on theday of vaccination were compared with samples taken at onset of COVID-19 associated symptoms.Long-read Nanopore PCR-cDNA sequencing technology was utilised.

Sample: sample13_CT_MenACWY_-ve

SAMN34058681 • SRS17214550 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: GSM7140112

Instrument: PromethION

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Peripheral blood (up to 2.5ml) was collected into a PAXgeneTM RNA tube at vaccination (D0) and at SARS-CoV-2 diagnosis (CT). Total RNA was extracted using the PAXgene blood RNA kit (PreAnalytiX, Switzerland) on the QIAsymphony SP (Qiagen) with the PAX_RNA V5 protocol. Eluted RNA was quantified using the Agilent RNA ScreenTape assay on the 4200 TapeStation System (Agilent). PCR-cDNA libraries were prepared using the SQK-PCS110 PCR-cDNA-sequencing kit following the standard Oxford Nanopore Technologies (ONT) protocol. Samples were barcoded (#1-8) and sequenced on PromethION. 2 samples were loaded per flow cell (15 ng each), with the paired samples run together and the unpaired samples randomly split into pairs

Runs: 1 run, 82M spots, 62.8G bases, 55.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR24055363	82,019,086	62.8G	55.3Gb	2024-03-04

ID:: 27226691

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