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SRX19732482: GSM7106146: open_chor_08h_ck27Ml_s512; Gallus gallus; RNA-Seq
5 ILLUMINA (Illumina NovaSeq 6000) runs: 32M spots, 3.2G bases, 963.5Mb downloads

External Id: GSM7106146_r1
Submitted by: Dept. of Biosciences and Disease, New England College of Optometry
Study: Daily Patterns of Retinal and Choroidal Gene Expression and Circadian Biology in Myopia Pathogenesis
PRJNA946718 • SRP428304 • All experiments • All runs
show Abstracthide Abstract
We investigated diurnal changes in gene expression in the retina and choroid at the onset of experimental myopia. We induced visual form deprivation myopia in young chicks, and isolated retinal and choroidal tissues separately in form- deprived and contralateral control eyes every 4 hours over a single 24-hour period. Overall design: After applying RNA-Seq separately to each tissue extract, we used various bioinformatics analyses and gene expression profiling analyses to assess the retinas and choroids of experimental and control eyes at each time point.
Sample: open_chor_08h_ck27Ml_s512
SAMN33831635 • SRS17099248 • All experiments • All runs
Organism: Gallus gallus
Library:
Name: GSM7106146
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: RNA was extracted from choroid and retinal tissues using the QIAgen RNAeasy kit Illumina TruSeq stranded kit using roughly equal amounts of RNA (between 100 and 300ng)
Runs: 5 runs, 32M spots, 3.2G bases, 963.5Mb
Run# of Spots# of BasesSizePublished
SRR239212967,337,089733.7M219.6Mb2023-12-20
SRR239212977,350,052735M220.1Mb2023-12-20
SRR239212986,050,150605M182.8Mb2023-12-20
SRR239212995,998,291599.8M182.1Mb2023-12-20
SRR239213005,250,614525.1M158.9Mb2023-12-20

ID:
27043467

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