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SRX19731185: GSM7107597: SIR_wtpromoter_V5Rap1 input rep1; Saccharomyces cerevisiae; ChIP-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 6.9M spots, 699.1M bases, 203.4Mb downloads

External Id: GSM7107597_r1
Submitted by: Rine, Molecular and Cell Biology, University of California, Berkeley
Study: Context dependent function of transcriptional regulator Rap1 in gene silencing and activation [ChIPseq I]
PRJNA946776 • SRP428262 • All experiments • All runs
show Abstracthide Abstract
As part of a study on the role of Rap1 at the promoters of HML and MAT in S cerevisiae, we endogenously tagged Rap1 with a 3xV5 tag and performed ChIPseq in a variety of genetic backgrounds including: silenced (SIR); unsilenced (sir1?/sir3?/sir4?); in strains witha wild type alpha2 promoter (wtpromoter); or with a point mutation at the promoter (mutpromoter). Overall design: 3xV5-Rap1 ChIPseq. Replicates are included for every sample.
Sample: SIR_wtpromoter_V5Rap1 input rep1
SAMN33830282 • SRS17097914 • All experiments • All runs
Organism: Saccharomyces cerevisiae
Library:
Name: GSM7107597
Instrument: Illumina NovaSeq 6000
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: PAIRED
Construction protocol: Cells were lysed by mechanical disruption in a bead beater. Chromatin was fragmented by sonication. All immunoprecipitations were performed using 50uL DynaBeads Protein G (ThermoFisher Scientific 10003D) magnetic beads coupled with 5uL monoclonal mouse-anti-V5 antibody (ThermoFisher Scientific R96025) DNA was purified using Qiagen spin column kit NEB Ultra II library prep kit
Runs: 1 run, 6.9M spots, 699.1M bases, 203.4Mb
Run# of Spots# of BasesSizePublished
SRR239199186,853,653699.1M203.4Mb2023-05-09

ID:
27042170

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