GSM7090873: hFB cells, nanoCAGE, DACPano, BR1; Homo sapiens; RNA-Seq - SRA

SRX19627071: GSM7090873: hFB cells, nanoCAGE, DACPano, BR1; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 24.7M spots, 3.3G bases, 1.5Gb downloads

External Id: GSM7090873_r1

Submitted by: Ting Wang Lab, Genetics, Washington University in St. Louis

Study: Epigenetic therapy activates TE-chimeric transcripts to provide additional source of antigens in glioblastoma stem cells

PRJNA942972 • SRP426514 • All experiments • All runs

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Epigenetic treatment induces TE-chimeric antigens in glioblastoma stem cells Overall design: Methylation profiling by high throughput sequencing

Sample: hFB cells, nanoCAGE, DACPano, BR1

SAMN33706424 • SRS16996937 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: GSM7090873

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: CAGE

Layout: PAIRED

Construction protocol: mRNA from GSCs and primary cells was extracted using Dynabeads mRNA DIRECT Purification Kit (ThermoFisher Scientific, 61011). We enriched for 5'-capped mRNA by digesting mRNA extraction with Terminator exonuclease digestion (Lucigen, TER51020) following manufacture's recommendations. We followed the standard nanoCAGE protocol (Salimullah et al., Cold Spring Harbor Protocol, 2011)

Runs: 1 run, 24.7M spots, 3.3G bases, 1.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR23794914	24,706,039	3.3G	1.5Gb	2024-06-05

ID:: 26924817

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