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SRX19626997: GSM7090821: NHA cells, WGBS, DACPano, BR1; Homo sapiens; Bisulfite-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 273.3M spots, 74.2G bases, 23.8Gb downloads

External Id: GSM7090821_r1
Submitted by: Ting Wang Lab, Genetics, Washington University in St. Louis
Study: Epigenetic therapy activates TE-chimeric transcripts to provide additional source of antigens in glioblastoma stem cells
show Abstracthide Abstract
Epigenetic treatment induces TE-chimeric antigens in glioblastoma stem cells Overall design: Methylation profiling by high throughput sequencing
Sample: NHA cells, WGBS, DACPano, BR1
SAMN33706476 • SRS16996863 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: GSM7090821
Instrument: Illumina NovaSeq 6000
Strategy: Bisulfite-Seq
Source: TRANSCRIPTOMIC
Selection: RANDOM
Layout: PAIRED
Construction protocol: We extracted genomic DNA with Quick-DNA Miniprep Kit (Zymo, D3024) We bisulfite converted 200-400 ng of DNA spiked with 0.5% lambda DNA using EZ DNA Methylation-Direct kit (Zymo, D5020). For WGBS, we processed the bisulfite- converted DNA with Accel-NGS Methyl-Seq DNA Library Kit (Swift Biosciences, 30024).
Runs: 1 run, 273.3M spots, 74.2G bases, 23.8Gb
Run# of Spots# of BasesSizePublished
SRR23794989273,302,88574.2G23.8Gb2024-06-05

ID:
26924743

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