GSM7083335: GSC-040815-C3; Homo sapiens; RNA-Seq - SRA

SRX19578453: GSM7083335: GSC-040815-C3; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 23.1M spots, 4.6G bases, 1.4Gb downloads

External Id: GSM7083335_r1

Submitted by: Population Health Sciences, UT Health Science Center at San Antonio

Study: Preclinical development of brain permeable ERß agonist for the treatment of glioblastoma

PRJNA941346 • SRP425936 • All experiments • All runs

show Abstracthide Abstract

We examined the mechanisms by estrogen receptor beta agonist CIDD-0149897 regulates glioma progression. Glioma stem cells (GSCs) were treated with CIDD-0149897 , and total RNA was isolated for RNA-seq analysis. Our results demonstrated that treatment with CIDD-0149897 showed upregulation of apoptotic and p53 pathways and downregulates several pathways including MYC and E2F. Overall design: Total RNA from vehicle (0.1% DMSO v/v) and CIDD-0149897 (20 µM) treated cells were isolated using RNeasy mini kit (Qiagen). Samples were prepared in triplicates and sequenced on the Illumina NovaSeq 6000 platform (Illumina Inc.) using the 150bp paired-end sequencing module.

Sample: GSC-040815-C3

SAMN33604223 • SRS16961432 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: GSM7083335

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA from vehicle (0.1% DMSO v/v) and CIDD-0149897 treated cells were isolated using RNeasy mini kit (Qiagen). Illumina TruSeq RNA Sample preparation and sequencing was done using UT Health San Antonio sequencing core protocol. RNA-seq library preparation by following the Illumina TruSeq stranded mRNA sample preparation guide

Runs: 1 run, 23.1M spots, 4.6G bases, 1.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR23717299	23,108,538	4.6G	1.4Gb	2023-09-18

ID:: 26871080

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