GSM7080328: Jiashi melon Control 6d, replicate 1; Cucumis melo; RNA-S... - SRA

SRX19560065: GSM7080328: Jiashi melon Control 6d, replicate 1; Cucumis melo; RNA-Seq
1 BGISEQ (BGISEQ-500) run: 22.8M spots, 1.1G bases, 981.9Mb downloads

External Id: GSM7080328_r1

Submitted by: Xinjiang Agricultural University, College of Food Science and Pharmacy

Study: Transcriptome sequencing of postharvest melon fruit infected by Alternaria alternata

PRJNA940813 • SRP425561 • All experiments • All runs

show Abstracthide Abstract

Jiashi melon and 86-1 melon were inoculated with Alternaria alternata, and the difference of gene expression was analyzed after 0, 6, 12, 18 and 24 days storage. Overall design: Jiashi melon and 86-1 melon were inoculated with Alternaria alternata (control inoculated with sterile water), and samples were collected at 0d, 6d, 12d, 18d and 24d after storage, respectively, and transcriptome sequencing was performed to compare gene expression differences. The experiment was set up with 3 replicates.

Sample: Jiashi melon Control 6d, replicate 1

SAMN33579670 • SRS16944530 • All experiments • All runs

Organism: Cucumis melo

Library:

Name: GSM7080328

Instrument: BGISEQ-500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: In total, approximately 1 μg total RNA was initially used for BGISEQ-500 library construction. In general, DNase I was initially used to degrade double-stranded and single-stranded DNA contaminant in RNA samples. The mRNA molecules were then purified from total RNA using oligo(dT)-attached magnetic beads and fragmented into small pieces. First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. The cDNA thus synthesized was subjected to end-repair and 3′ adenylation. Subsequently, adaptors were ligated to the ends of these 3′ adenylated cDNA fragments. The double stranded PCR products were heat denatured and circularized by the splint oligo sequence. The single stranded circular DNAs were formatted as the final library for Agilent Technologies 2100 bioanalyzer validation and subsequent SE50 sequencing. RNA libraries were prepared for sequencing using BGISEQ-500 platform

Runs: 1 run, 22.8M spots, 1.1G bases, 981.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR23698102	22,761,022	1.1G	981.9Mb	2024-03-01

ID:: 26848747

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