GSM7036304: EC_H1_2; Drosophila melanogaster; OTHER - SRA

SRX19323667: GSM7036304: EC_H1_2; Drosophila melanogaster; OTHER
1 ILLUMINA (Illumina HiSeq 2500) run: 40.7M spots, 2.1G bases, 503.9Mb downloads

External Id: GSM7036304_r1

Submitted by: Institut Curie

Study: Chromatin state transitions in the Drosophila intestinal lineage reveal principles of cell type specification [DamID]

PRJNA934539 • SRP422586 • All experiments • All runs

show Abstracthide Abstract

To investigate the chromatin state into an adult stem cell lineage, we generate cell-type specific chromatin state maps in the adult Drosophila intestine and identify principal chromatin state transitions during lineage determination. we profiled the binding sites of five chromatin-associated proteins from which the previously described five major types of chromatin. Overall design: Comparative chromatin accessibility (ATAC-seq) as well as gene expression (RNA-seq) in Drosophila ISC/EB in control and H1 RNAi conditions

Sample: EC_H1_2

SAMN33228632 • SRS16721649 • All experiments • All runs

Organism: Drosophila melanogaster

Library:

Name: GSM7036304

Instrument: Illumina HiSeq 2500

Strategy: OTHER

Source: GENOMIC

Selection: other

Layout: SINGLE

Construction protocol: For each replicate, DNA was extracted from 60 dissected guts via Qiagen QIAmp DNA Micro Kit following manufacturers' instructions, Extracted DNA was digested with DpnI, ligated with DamID adaptors, digested with DpnII before PCR amplification. DNA was sonicated to 300bp, adaptors were removed with AlwI and end-repaired and A-tailed. Sequencing adaptors were ligated to the fragments before PCR amplification.

Runs: 1 run, 40.7M spots, 2.1G bases, 503.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR23385465	40,742,591	2.1G	503.9Mb	2023-10-26

ID:: 26572203

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