GSM7035161: Cpa3Cre/+, naive, rep2 [AS-929274]; Mus musculus; RNA-Seq - SRA

SRX19319792: GSM7035161: Cpa3Cre/+, naive, rep2 [AS-929274]; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 1000) run: 27.2M spots, 6G bases, 1.8Gb downloads

External Id: GSM7035161_r1

Submitted by: Cellular Immunology, Immunology, German Cancer Research Center

Study: Mast cells link immune sensing to antigen avoidance behavior [intestine]

PRJNA933010 • SRP421630 • All experiments • All runs

show Abstracthide Abstract

We investigated antigen-specific avoidance behavior in mice, and found that is critically dependent on mast cells. We analyzed gene expression comparing naive versus immunized wild type mice, and versus mast cell-deficient mice at different times following antigen exposure Overall design: Comparative gene expression profiling analysis of RNA-seq data for Cpa3+/+ (wild type) and Cpa3Cre/+ (mast cell-deficient) mice

Sample: Cpa3Cre/+, naive, rep2 [AS-929274]

SAMN33219709 • SRS16718222 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM7035161

Instrument: NextSeq 1000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was isolated from whole Jejunum (fat and Peyer's plaques removed) according to the instructions of the PureLink RNA Mini kit (Invitrogen) RNA libraries for RNA-seq were prepared using TruSew Stranded RNA Kit (Illumina) following manufacturer's protocols.

Runs: 1 run, 27.2M spots, 6G bases, 1.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR23379175	27,191,245	6G	1.8Gb	2023-04-11

ID:: 26567987

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