GSM7027222: BW25113 wt, ADRPC+, rep1; Escherichia coli; RNA-Seq - SRA

SRX19290085: GSM7027222: BW25113 wt, ADRPC+, rep1; Escherichia coli; RNA-Seq
1 ILLUMINA (NextSeq 2000) run: 39.6M spots, 4.8G bases, 1.1Gb downloads

External Id: GSM7027222_r1

Submitted by: Institute Of Biotechnology, Life Sciences Center, Vilnius University

Study: 5'-NAD-RNA identification in E. coli BW25113 wt, nudC and csdA knockouts

PRJNA934575 • SRP424182 • All experiments • All runs

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In the present study we applied NAD captureSeq to charecterize the 5'-NAD-RNA profile of E. coli BW25113 wt strain as well as nudC and csdA knockouts. The data obtained showed that both knockouts resulted in an increased number of identified 5'-NAD-RNA species, thus suggesting that CsdA might add a potentially new layer of control on NAD-epitranscriptomic landscape. Overall design: 5'-NAD-RNA identification in E. coli BW25113 wt, nudC KO and csdA KO strains. 3 Biological replicates for ADRPC+ and 2 biological replicates for ADRPC- libraries.

Sample: BW25113 wt, ADRPC+, rep1

SAMN33138969 • SRS16691101 • All experiments • All runs

Organism: Escherichia coli

Library:

Name: GSM7027222

Instrument: NextSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was extracted using RNazol RT (RN 190) (Molecular Research Center, Inc.) according to the manufacturer's recommendations. NAD captureSeq libraries were prepared according to the modified protocol provided by (Winz et al. 2017).

Runs: 1 run, 39.6M spots, 4.8G bases, 1.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR23348268	39,598,120	4.8G	1.1Gb	2023-08-22

ID:: 26526872

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