GSM7000653: Haloferax_mediterranei_3; Haloferax mediterranei; OTHER - SRA

SRX19205910: GSM7000653: Haloferax_mediterranei_3; Haloferax mediterranei; OTHER
1 ILLUMINA (NextSeq 500) run: 2.3M spots, 681.4M bases, 297.8Mb downloads

External Id: GSM7000653_r1

Submitted by: Danko and Lis labs, Molecular Biology and Genetics, Cornell University

Study: Pol II pausing is a milestone on the road to complex animals

PRJNA929029 • SRP419683 • All experiments • All runs

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PRO-seq experiments in multiple organisms. Also, PRO-seq experiment of Nelfb and Nelfe degron mESC in serum/LIF with or without dTAG-13. Overall design: We used Precision Run-On and Sequencing (PRO-seq) to study transcription in 20 extant organisms that represent very diverse branches on the tree of life, including multiple species near the base of the Metazoa phylogeny and the transition between plants and animals. We also used PRO-seq to study mESCs after degrading NELF-B or NELF-E.

Sample: Haloferax_mediterranei_3

SAMN32949473 • SRS16614670 • All experiments • All runs

Organism: Haloferax mediterranei

Library:

Name: GSM7000653

Instrument: NextSeq 500

Strategy: OTHER

Source: TRANSCRIPTOMIC

Selection: other

Layout: PAIRED

Construction protocol: RNA extraction, run-on, and library preparation were executed according to “Mahat, D. B. et al. Base-pair-resolution genome-wide mapping of active RNA polymerases using precision nuclear run-on (PRO-seq). Nat. Protoc. 11, 1455–1476 (2016).” (PMID: 27442863). Library prep was performed using Illumina custom adapters: Reverse 3' RNA adapter (/5Phos/NNNNNNGAUCGUCGGACUGUAGAACUCUGAAC/3InvdT) and Reverse 5' RNA Adapter (5'-CCUUGGCACCCGAGAAUUCCA-3').

Runs: 1 run, 2.3M spots, 681.4M bases, 297.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR23260653	2,271,465	681.4M	297.8Mb	2024-02-05

ID:: 26407905

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