GSM6807051: RNA_edc3scd6_Replicate_2; Saccharomyces cerevisiae; RNA-S... - SRA

SRX18543682: GSM6807051: RNA_edc3scd6_Replicate_2; Saccharomyces cerevisiae; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 67.7M spots, 6.8G bases, 1.9Gb downloads

External Id: GSM6807051_r1

Submitted by: Section on Nutrient Control of Gene Expression, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health

Study: Decapping factor Dcp2 controls mRNA abundance and translation to adjust metabolism and filamentation to nutrient availability II

PRJNA910205 • SRP412088 • All experiments • All runs

show Abstracthide Abstract

Degradation of most yeast mRNAs involves decapping by Dcp1/Dcp2. DEAD-box protein Dhh1 has been implicated as an activator of decapping, and as a translational repressor, but their functions in cells are incompletely understood. We have analyzed these questions by a combination of ribosome profiling, RNA-Seq, CAGE analysis of capped mRNAs. Overall design: It includes total 14 samples. pat1, pat1dhh1 (triplicates) and edc3scd6 (duplicates) were subjected to RNA-Seq. Separately, WT and dcp2 in triplicates (6 samples) were subjected to Rpb1 ChIP-seq. All strains were grown in YPD at 30ºC till the OD600 ~0.6

Sample: RNA_edc3scd6_Replicate_2

SAMN32118014 • SRS16009911 • All experiments • All runs

Organism: Saccharomyces cerevisiae

Library:

Name: GSM6807051

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: For RNA-seq library preparation, total RNA was extracted and purified from aliquots of the same snapped-frozen cells described above using Zymo RNA Clean and Concentrator kit. and the library was prepared by NHLBI Sequencing and Genomics Core at NIH (Bethesda, MD) using TruSeq Stranded mRNA Library Prep Kit (Illumina) In additional columns of the SAMPLES section In additional columns of the SAMPLES section

Runs: 1 run, 67.7M spots, 6.8G bases, 1.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR22580678	67,665,181	6.8G	1.9Gb	2023-02-07

ID:: 25598051

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