GSM6768163: Pilot 1 2.0mm 30s; Zea mays; RNA-Seq - SRA

SRX18433953: GSM6768163: Pilot 1 2.0mm 30s; Zea mays; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 153,944 spots, 21.8M bases, 10.3Mb downloads

External Id: GSM6768163_r1

Submitted by: Walbot, Biology, Stanford University

Study: The establishment of the anther somatic niche with single cell sequencing

PRJNA907016 • SRP410686 • All experiments • All runs

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Maize anthers ranging in size from 0.5mm to 3.0mm in increments of 0.25mm were dissected and their cells isolated using the FX-Cell protocol. scRNA-seq libraries were prepped using the CEL-Seq2 protocol and sequenced with 150bp PE reads with Illumina HiSeq or NovoSeq. The developmental trajectories of the somatic cell layers were investigated. Overall design: Anthers were dissected from maize plants of the fertile inbred line W23. Anthers ranging in size from 0.5mm to 3.0mm in increments of 0.25mm were dissected, isolated using a Hana Cell Sorter (NamoCell) and used for scRNA-seq. At least two separate W23 plants provided anthers for each stage

Sample: Pilot 1 2.0mm 30s

SAMN31948118 • SRS15915689 • All experiments • All runs

Organism: Zea mays

Library:

Name: GSM6768163

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC SINGLE CELL

Selection: cDNA

Layout: SINGLE

Construction protocol: Anthers dissected, fixed, digested, isolated using a Hana Cell Sorter (NamoCell) following FX-Cell protocol (Marchant et al. 2022) CEL-Seq2 (Hashimshony et al., 2016) with modifications per Nelms and Walbot 2019.

Runs: 1 run, 153,944 spots, 21.8M bases, 10.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR22467669	153,944	21.8M	10.3Mb	2023-01-01

ID:: 25462099

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