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SRX18433673: GSM6768035: 7.21 1 2.0mm 10s; Zea mays; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 29,535 spots, 3.1M bases, 2.2Mb downloads

External Id: GSM6768035_r1
Submitted by: Walbot, Biology, Stanford University
Study: The establishment of the anther somatic niche with single cell sequencing
show Abstracthide Abstract
Maize anthers ranging in size from 0.5mm to 3.0mm in increments of 0.25mm were dissected and their cells isolated using the FX-Cell protocol. scRNA-seq libraries were prepped using the CEL-Seq2 protocol and sequenced with 150bp PE reads with Illumina HiSeq or NovoSeq. The developmental trajectories of the somatic cell layers were investigated. Overall design: Anthers were dissected from maize plants of the fertile inbred line W23. Anthers ranging in size from 0.5mm to 3.0mm in increments of 0.25mm were dissected, isolated using a Hana Cell Sorter (NamoCell) and used for scRNA-seq. At least two separate W23 plants provided anthers for each stage
Sample: 7.21 1 2.0mm 10s
SAMN31948246 • SRS15915409 • All experiments • All runs
Organism: Zea mays
Library:
Name: GSM6768035
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC SINGLE CELL
Selection: cDNA
Layout: SINGLE
Construction protocol: Anthers dissected, fixed, digested, isolated using a Hana Cell Sorter (NamoCell) following FX-Cell protocol (Marchant et al. 2022) CEL-Seq2 (Hashimshony et al., 2016) with modifications per Nelms and Walbot 2019.
Runs: 1 run, 29,535 spots, 3.1M bases, 2.2Mb
Run# of Spots# of BasesSizePublished
SRR2246743829,5353.1M2.2Mb2023-01-01

ID:
25461819

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