GSM6753440: N8 NRK-52E cells, pH6; Rattus norvegicus; RNA-Seq - SRA

SRX18360544: GSM6753440: N8 NRK-52E cells, pH6; Rattus norvegicus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 26.1M spots, 7.8G bases, 2.5Gb downloads

External Id: GSM6753440_r1

Submitted by: Julius Bernstein Institute for Physiology, Martin Luther University - Halle-Wittenberg

Study: Acidosis activates the Nrf2-pathway in renal proximal tubule-derived cells through a crosstalk with renal fibroblasts

PRJNA904711 • SRP409422 • All experiments • All runs

show Abstracthide Abstract

Crosstalk of renal epithelial cells with interstitial fibroblasts plays an important role in kidney pathophysiology. A former study showed that crosstalk between renal epithelial cells and renal fibroblasts protects against acidosis-induced damage. In order to gain further mechanistic insight into this crosstalk we investigated the effect of acidosis on the transcriptome of renal epithelial cells (NRK-52E) and renal fibroblasts (NRK-49F) in co-culture by RNASeq, bioinformatics analysis and experimental validation. Overall design: NRK-52E (proximal tubule-derived epithelial cells) and NRK-49F (kidney fibroblasts) were subjected to acidic conditions, while being co-cultivated.

Sample: N8 NRK-52E cells, pH6

SAMN31851373 • SRS15847196 • All experiments • All runs

Organism: Rattus norvegicus

Library:

Name: GSM6753440

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was isolated with BluZol Reagent, following the manufacturer's instructions. Eventual gDNA contaminations were removed using Turbo DNAse (ambion, Life Technologies). polyA enrichment (performed by Novogene Europe)

Runs: 1 run, 26.1M spots, 7.8G bases, 2.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR22390529	26,123,382	7.8G	2.5Gb	2023-02-27

ID:: 25375134

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