GSM6710882: dCas9-KRAB DF1, Mock control_A; Gallus gallus; RNA-Seq - SRA

SRX18173620: GSM6710882: dCas9-KRAB DF1, Mock control_A; Gallus gallus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 38.7M spots, 11.6G bases, 3.4Gb downloads

External Id: GSM6710882_r1

Submitted by: Penn State University

Study: Targeted modulation of chicken genes in vitro using CRISPRa and CRISPRi toolkit

PRJNA898374 • SRP406343 • All experiments • All runs

show Abstracthide Abstract

To investigate the effectiveness of CRISPR based gene modulation, IRF7 was repressed in dCas9-KRAB DF1 cell line or activated in dCas9-VPR DF1 cell line followed by whole transcriptome analysis Overall design: a. Differntiall gene expression analysis between mock (non-targeting) and IRF7 targeting gRNAs transfected dCas9-KRAB DF1 cells for assessing the endogenous targeted gene knockdown b. Differntiall gene expression analysis between mock (non-targeting) and IRF7 targeting gRNAs transfected dCas9-VPR DF1 cells for assessing the endogenous targeted gene activation

Sample: dCas9-KRAB DF1, Mock control_A

SAMN31610275 • SRS15672842 • All experiments • All runs

Organism: Gallus gallus

Library:

Name: GSM6710882

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was extracted using Zymo DirectZol mini mRNA library preparation (poly A enrichment)

Runs: 1 run, 38.7M spots, 11.6G bases, 3.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR22195297	38,667,541	11.6G	3.4Gb	2022-11-07

ID:: 25156489

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