GSM6668360: Human/chimpanzee tetraploid hybrid 21 day embrypoid bodie... - SRA

SRX17989590: GSM6668360: Human/chimpanzee tetraploid hybrid 21 day embrypoid bodies - replicate 3, 10x lane 2; Pan troglodytes; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 856.6M spots, 185.2G bases, 55.1Gb downloads

External Id: GSM6668360_r1

Submitted by: Yoav Gilad Lab, Genetic Medicine, University of Chicago

Study: The relationship between regulatory changes in cis and trans and the evolution of gene expression in humans and chimpanzees

PRJNA832107 • SRP372276 • All experiments • All runs

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We used embryoid bodies to compare differences in gene expression between humans and chimpanzees in a large number of cell types Overall design: Single cell RNA-sequencing of human and chimpanzee embryoid bodies. We generated embryoid bodies from 3-6 human and 3 chimpanzee inividuals in three replicates. For each replicate, embryoid bodies from both species were dissociated and pooled prior to collecting ~40,000-60,000 cells with 10x scRNA-seq. Single cell RNA-sequencing of human/chimpanzee tetraploid hybrid embryoid bodies. We generated embryoid bodies from one hybrid inividuals in three replicates. For each replicate, we collected ~20,000 cells and performed 10x 3' scRNA-seq v3.1.

Sample: Human/chimpanzee tetraploid hybrid 21 day embrypoid bodies - replicate 3, 10x lane 2

SAMN31405652 • SRS15504043 • All experiments • All runs

Organism: Pan troglodytes

Library:

Name: GSM6668360

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC SINGLE CELL

Selection: cDNA

Layout: PAIRED

Construction protocol: EBs were dissocated in Accumax, pooled, and collected on a 10x Chromium controller. Libraries were constructed using 10x 3' scRNA-seq v3.1 according to manufacturer directions.

Runs: 1 run, 856.6M spots, 185.2G bases, 55.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR22007287	856,552,193	185.2G	55.1Gb	2023-04-11

ID:: 24941460

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