SRA

To investegate the mechanism of physiological changes during cell digestion, we analyzed the levels of RNAs in cells treated with animal-based enzymes and animal origin-free enzymes. Overall design: The Vero cells were divided into 3 groups. The first group (Group 1) was digested with trypsin at 37°C. The cells collected immediately after digestion were set to 0 h, and the digested cells were placed back in the cell incubator for 8 hours. After the cells were digested with non-enzymatic digestion solution and then collected for 8 h, the cells af-ter trypsinization at 37°C were further cultured for 24 hours, and the cells were di-gested with non-enzymatic digestion solution and collected for 24 h. The second group (Group 2) was digested with TrypLE at 37°C, then set the cells collected immediately after digestion as 0 h, place the digested cells back in the cell incubator for 8 hours, and digest the recovered cells with non-enzyme digestion solution. Set it to 8h, continue to culture the cells digested with TrypLE digestion solution at 37°C for 24 hours, and then digest the cells with non-enzyme digestion solution for 24h. The third group (Group 3) used 4°C TrypLE digestion solution at 25°C digestion, the cells collected immediately after digestion were set to 0 h, and the digested cells were placed back in the cell incu-bator for 8 hours, and the cells collected after digestion with non-enzyme digestion solution were set to 8 h, and digested with TrypLE digestion solution at 4°C. After the cells were cultured for 24 hours, the collected cells were digested with non-enzymatic digestion solution and set to 24 hours. In addition, Vero cells were digested with an enzyme-free digestion solution at 37°C, and the immediately harvested cells were set as the control group. Each sample has three biological replicates.