GSM6620204: ATAC_V2; Mus musculus; ATAC-seq - SRA

SRX17827924: GSM6620204: ATAC_V2; Mus musculus; ATAC-seq
1 ILLUMINA (NextSeq 500) run: 72.8M spots, 5.5G bases, 1.9Gb downloads

External Id: GSM6620204_r1

Submitted by: Pu lab, Cardiology, Boston Children's Hospital

Study: Transposase-accessible chromatin with high-throughput sequencing for purified neonatal aCMs and vCMs

PRJNA888106 • SRP401529 • All experiments • All runs

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Examination of chamber selective chromatin accessibility and investigate if chromatin accessibility regulates CSEs. Overall design: Neonatal atrial and ventricular cardiomyocytes were isolated and purified for ATAC-seq to identify the chamber selective chromatin accessibility regions.

Sample: ATAC_V2

SAMN31208371 • SRS15351321 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM6620204

Instrument: NextSeq 500

Strategy: ATAC-seq

Source: GENOMIC

Selection: other

Layout: SINGLE

Construction protocol: The optimized-ATACSeq (omni-ATACSeq) libraries were generated from the isolated cardiomyocytes (two biological replicates) using the author's recommended lysis steps and transposition protocol. Corces MR, et al. Nat Methods. 2017. The DNA fragments were amplified with NEBNext High-fidelity 2x PCR master mix. Purify amplified library using Qiagen MinElute PCR Purification kit.

Runs: 1 run, 72.8M spots, 5.5G bases, 1.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR21838986	72,802,272	5.5G	1.9Gb	2023-01-31

ID:: 24755046

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