GSM6523465: TuMV biol rep 3; Arabidopsis thaliana; RNA-Seq - SRA

SRX17360610: GSM6523465: TuMV biol rep 3; Arabidopsis thaliana; RNA-Seq
1 ILLUMINA (Illumina MiSeq) run: 458,561 spots, 69.7M bases, 38.8Mb downloads

External Id: GSM6523465_r1

Submitted by: IBMP, CNRS

Study: Diversity of phytoviral RNA U-tailing among ss(+) RNA phytoviruses

PRJNA875112 • SRP394922 • All experiments • All runs

show Abstracthide Abstract

Using 3' RACE-seq method, we analyzed global 3' terminal RNA uridylation profiles for representatives of the main families of positive single-stranded RNA phytoviruses. Overall design: 3' RACE-seq method was used to measure poly(A) tail sizes and analyze uridylation levels. This protocol is based on the ligation of a primer at the 3' end of RNA, and the subsequent targeted amplification by PCR of amplicons suitable for Illumina sequencing.

Sample: TuMV biol rep 3

SAMN30601002 • SRS14919962 • All experiments • All runs

Organism: Arabidopsis thaliana

Library:

Name: GSM6523465

Instrument: Illumina MiSeq

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RACE

Layout: PAIRED

Construction protocol: Total RNA from infected leaves of C. quinoa, N. benthamiana and grapevine were extracted using the RNeasy plant Mini Kit (Qiagen). Total RNA of Arabidopsis infected leaves was extracted using Tri-Reagent (Molecular Research Center). 3' RACE-seq 3'RACEseq protocol is based on the ligation of a primer at the 3' end of RNA, and the subsequent targeted amplification by PCR of amplicons suitable for Illumina sequencing.

Runs: 1 run, 458,561 spots, 69.7M bases, 38.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR21354849	458,561	69.7M	38.8Mb	2023-04-25

ID:: 24181733

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity