GSM6528551: GFLV_B844_RNA1&2 V. vinifera_Plant3; Vitis vinifera; RNA-... - SRA

SRX17360578: GSM6528551: GFLV_B844_RNA1&2 V. vinifera_Plant3; Vitis vinifera; RNA-Seq
1 ILLUMINA (Illumina MiSeq) run: 339,585 spots, 51.6M bases, 28.4Mb downloads

External Id: GSM6528551_r1

Submitted by: IBMP, CNRS

Study: RNA uridylation percentages for virus of the Secoviridae family

PRJNA875114 • SRP394921 • All experiments • All runs

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Using 3' RACE-seq method, we analyzed global 3' terminal RNA uridylation profiles of Secovirus RNAs. Overall design: 3' RACE-seq method was used to measure poly(A) tail sizes and analyze uridylation levels. This protocol is based on the ligation of a primer at the 3' end of RNA, and the subsequent targeted amplification by PCR of amplicons suitable for Illumina sequencing.

Sample: GFLV_B844_RNA1&2 V. vinifera_Plant3

SAMN30601074 • SRS14919930 • All experiments • All runs

Organism: Vitis vinifera

Library:

Name: GSM6528551

Instrument: Illumina MiSeq

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RACE

Layout: PAIRED

Construction protocol: Total RNA from infected leaves were extracted using the RNeasy plant Mini Kit (Qiagen). 3'RACE-seq 3'RACEseq protocol is based on the ligation of a primer at the 3' end of RNA, and the subsequent targeted amplification by PCR of amplicons suitable for Illumina sequencing.

Runs: 1 run, 339,585 spots, 51.6M bases, 28.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR21354815	339,585	51.6M	28.4Mb	2023-04-25

ID:: 24181701

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Sequence Read Archive

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