GSM6523480: GFLV_urt1_1_Plant3; Arabidopsis thaliana; RNA-Seq - SRA

SRX17360481: GSM6523480: GFLV_urt1_1_Plant3; Arabidopsis thaliana; RNA-Seq
1 ILLUMINA (Illumina MiSeq) run: 427,329 spots, 65M bases, 35.6Mb downloads

External Id: GSM6523480_r1

Submitted by: IBMP, CNRS

Study: Involvement of Arabidopsis TUTases in viral RNA uridylation

PRJNA875113 • SRP394920 • All experiments • All runs

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Using 3' RACE-seq method, we analyzed the impact of URT1 and/or HESO1 inactivation on the uridylation of GFLV, TuMV and TCV RNAs in Arabidopsis. Overall design: 3' RACE-seq method was used to measure poly(A) tail sizes and analyze uridylation levels. This protocol is based on the ligation of a primer at the 3' end of RNA, and the subsequent targeted amplification by PCR of amplicons suitable for Illumina sequencing.

Sample: GFLV_urt1_1_Plant3

SAMN30600985 • SRS14919833 • All experiments • All runs

Organism: Arabidopsis thaliana

Library:

Name: GSM6523480

Instrument: Illumina MiSeq

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RACE

Layout: PAIRED

Construction protocol: Total RNA of Arabidopsis infected leaves was extracted using Tri-Reagent (Molecular Research Center). 3'RACEseq 3'RACEseq protocol is based on the ligation of a primer at the 3' end of RNA, and the subsequent targeted amplification by PCR of amplicons suitable for Illumina sequencing.

Runs: 1 run, 427,329 spots, 65M bases, 35.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR21354785	427,329	65M	35.6Mb	2023-04-25

ID:: 24181604

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