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SRX17213616: GSM6507503: ST3, Control, Day 7, rep 3; Homo sapiens; RNA-Seq
4 ILLUMINA (NextSeq 500) runs: 5.2M spots, 394.9M bases, 176.7Mb downloads

External Id: GSM6507503_r1
Submitted by: Ghent University
Study: Transcriptome analysis of in vitro hematopoietic stem cells (HSC) and stage 3 (ST3) NK cell progenitors cells expressing a TXNIP-specific shRNA.
PRJNA873583 • SRP394013 • All experiments • All runs
show Abstracthide Abstract
To investigate the function of TXNIP in the regulation of natural killer (NK) cell differentiation, we established in vitro NK cell differentation cultures starting with cordblood derived HSC in which TXNIP has been knocked down with shRNA by lentiviral transduction. We then performed transcriptome analysis of the HSC population at day 3 and ST3 population at day 7. Overall design: mRNA profile of HSC (d3) and ST3 (d7) transduced with shTXNIP in comparison to control transduced cells
Sample: ST3, Control, Day 7, rep 3
SAMN30496935 • SRS14780834 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: GSM6507503
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total mRNA was extracted using the RNeasy micro kit (Qiagen). The concentration and quality of the total extracted RNA was checked by using the Quant-it ribogreen RNA assay (Life Technologies) and the RNA 6000 nano chip (Agilent Technologies), respectively. Illumina sequencing library preparation was performed using the QuantSeq 3' mRNA-Seq Library Prep Kits (Lexogen, Vienna, Austria) according to manufacturer's protocol. Libraries were quantified by qPCR.
Runs: 4 runs, 5.2M spots, 394.9M bases, 176.7Mb
Run# of Spots# of BasesSizePublished
SRR212027151,343,244101.2M44.8Mb2022-10-31
SRR212027161,314,68799.1M44Mb2022-10-31
SRR212027171,293,16597.4M43.9Mb2022-10-31
SRR212027181,289,89197.2M43.9Mb2022-10-31

ID:
24030750

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