GSM6472949: MARCM yki3SA sd 3; Drosophila melanogaster; RNA-Seq - SRA

SRX17116755: GSM6472949: MARCM yki3SA sd 3; Drosophila melanogaster; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 19.3M spots, 1.8G bases, 595.6Mb downloads

External Id: GSM6472949_r1

Submitted by: Laboratory of Growth Control, VIB-KU Leuven Center for Cancer Biology, VIB-KU Leuven

Study: Hippo signaling instructs ectopic but not normal organ growth [Drosophila YkiHyper]

PRJNA870447 • SRP392680 • All experiments • All runs

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In order to study how ectopic Yki drives tissue overgrowth in Drosophila imaginal discs, we overexpressed the constitutively active Yki3SA and deleted wts in clones of cells in the entire eye-antennal imaginal disc, as well as specifically in eye disc proper cells using Optix-Gal4. Using the MARCM system allowed us to compare the effects of Yki3SA overexpression in wild-type and sd mutant clones. Overall design: Differential gene expression analysis of samples of eye-antennal imaginal discs with Yki hyperactivation and their corresponding controls: 1) eyFLP wts KO and eyFLP control; 2) optix>Yki3SA and optix control; 3) eyFLP MARCM Yki3SA and eyFLP MARCM ctrl as well as eyFLP MARCM Yki3SA sd- and eyFLP MARCM sd- to measure the effects of Yki hyperactivation in sd mutant clones.

Sample: MARCM yki3SA sd 3

SAMN30370806 • SRS14690904 • All experiments • All runs

Organism: Drosophila melanogaster

Library:

Name: GSM6472949

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Drosophila imaginal discs were dissected and transferred into RNAse-free 1xPBS on ice; a minimum of 30 discs per sample were used. RNA was extracted immediately using the RNAeasy mini spin kit (Qiagen) and stored at -80°C. Library preparation was performed according to the QuantSeq 3' mRNA-Seq Library Prep kit FWD (Lexogen).

Runs: 1 run, 19.3M spots, 1.8G bases, 595.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR21103209	19,286,967	1.8G	595.6Mb	2022-11-18

ID:: 23916026

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Sequence Read Archive

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