GSM2125274: HepG2 cells expressing ATP7B H1069Q and treated with JNK ... - SRA

SRX1710758: GSM2125274: HepG2 cells expressing ATP7B H1069Q and treated with JNK Inhibitor, biological rep2; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 1000) run: 29.5M spots, 5.8G bases, 3.7Gb downloads

Submitted by: NCBI (GEO)

Study: Transcriptome profile of HepG2-expressing ATP7B-H1069Q (liver hepatocellular cells) exposed to JNK or p38 Inhibitor

PRJNA318738 • SRP073426 • All experiments • All runs

show Abstracthide Abstract

In order to identify the effects of JNK inhibitor or p38 Inhibitor on the transcriptome of ATP7B H1069Q-overexpressing liver cells, we performed RNAseq experiments Overall design: Transcriptome analysis of the hepatocytes overexpressing ATP7B H1069Q, compared with hepatocytes overexpressing ATP7B WT. Total RNA was extracted from HepG2 cells; RNA extracted from hepatocytes overexpressing ATP7B WT was used as control.

Sample: HepG2 cells expressing ATP7B H1069Q and treated with JNK Inhibitor, biological rep2

SAMN04866875 • SRS1400873 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 1000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Extraction of total RNA was performed according to the manufacturer's instructions (Qiagen). Total RNA (500 ng) from each sample was prepared using TruSeq RNA sample prep reagents (Illumina, San Diego, CA, USA) according to manufacturer's instructions The amplified fragmented cDNA of ∼200 bp in size were sequenced in paired-end mode using the HiSeq 2000 (Illumina) with a read length of 2×100 bp

Experiment attributes:

GEO Accession: GSM2125274

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 29.5M spots, 5.8G bases, 3.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR3399422	29,516,606	5.8G	3.7Gb	2017-01-04

ID:: 2448653

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