GSM2120843: sRNA_Ago1KO1; Mus musculus; ncRNA-Seq - SRA

SRX1700568: GSM2120843: sRNA_Ago1KO1; Mus musculus; ncRNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 23.5M spots, 1.2G bases, 442.1Mb downloads

Submitted by: NCBI (GEO)

Study: Small RNA profiling of WT E14, Ago2_KO and Ago1_KO mouse embryonic stem cells before and after XEN conversion and small RNA profiling after Immunoprecipitation of AGO1&2 proteins in WT E14, Ago1_KO and Ago2_KO mESCs.

PRJNA318281 • SRP073204 • All experiments • All runs

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Purpose: The goals of this study was to obtain the profile total miRNAs in WT E14, Ago2_KO and Ago1_KO mESCs to compare it with Immunoprecipitated microRNAs in AGO1&2 proteins. Overall design: ALL small RNA profiles from total RNA or after immunoprecipitation were generated by deep sequencing, in duplicate, using Illumina HiSeq2000. Small RNA profiling in Dicer_KO mESCs as been perform as a control library.

Sample: sRNA_Ago1KO1

SAMN04687748 • SRS1393716 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2000

Strategy: ncRNA-Seq

Source: TRANSCRIPTOMIC

Selection: size fractionation

Layout: SINGLE

Construction protocol: RNA was harvested using Trizol reagent. Illumina TruSeq Small RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries and 20-100 ng for the IP libraries. Small RNA libraries were prepared for sequencing using standard Illumina protocols by the Fasteris company (Switzerland). small RNA-Seq

Experiment attributes:

GEO Accession: GSM2120843

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 23.5M spots, 1.2G bases, 442.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR3370946	23,501,986	1.2G	442.1Mb	2018-02-05

ID:: 2435550

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