show Abstracthide AbstractTo investigate the key molecular and mechanism during the proliferation and differentiation of human aortic smooth muscle cells, we treated HASMC cell lines with PDGF, TGF-ß and miR-221.We then performed gene expression profiling analysis using data obtained from ONT long-read RNA-seq of 4 different treatment. Overall design: Comparative gene expression profiling analysis of RNA-seq data for control HASMC cells and 3 different treatments (PDGF, TGF-ß and miR-221).