GSM2096538: o2 rep2; Zea mays; RNA-Seq - SRA

SRX1655622: GSM2096538: o2 rep2; Zea mays; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 23.5M spots, 5.9G bases, 2.4Gb downloads

Submitted by: NCBI (GEO)

Study: Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes for Wild Type and Mutants including opaque2, PbfRNAi and PbfRNAi;o2 in Maize (Zea mays)

PRJNA316069 • SRP072207 • All experiments • All runs

show Abstracthide Abstract

The goals of this study are to study the regulatory network of the two maize endosperm-specific transcription factors O2 and PBF by 16-DAP endosperm transcriptome profiling (RNA-seq) of their mutants and wild type. The results utilize the expression pattern of global genes regulated by PBF and O2 to elucidate their control for storage compounds synthesis in maize kernels. Overall design: The 16-DAP endosperm transcriptome of wild type (WT) and mutants including opaque2, PbfRNAi and PbfRNAi;o2 were generated by RNA-seq with three biological replicates per genotype on Illumina HiSeqTM2500.

Sample: o2 rep2

SAMN04574810 • SRS1356001 • All experiments • All runs

Organism: Zea mays

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA are extracted from 16-DAP endosperm using TRIzol reagent (Invitrogen) and purified with the RNeasy Mini Kit (Qiagen). The mRNA are captured by Epicenter Ribo-Zero™ Magnetic Kit (No. MRZPL116-6). The libraries are prepared according Illumina Standard library preparation kit (Illumina USA) and sequenced to generate 125nt paired-end reads on Illumina HiSeqTM2500

Experiment attributes:

GEO Accession: GSM2096538

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 23.5M spots, 5.9G bases, 2.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR3284480	23,467,117	5.9G	2.4Gb	2016-03-25

ID:: 2374321

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity