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SRX16367705: GSM6364316: HBEC3 cells, TET3KO2; Homo sapiens; Bisulfite-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 665.7M spots, 171.2G bases, 66.4Gb downloads

Submitted by: NCBI (GEO)
Study: Dysfunction of 5-methylcytosine oxidases and the Polycomb protein RYBP leads to widespread DNA hypermethylation and cell transformation [WGBS]
PRJNA860621 • SRP387319 • All experiments • All runs
show Abstracthide Abstract
We inactivated RYBP, YAF2, TET1, TET2, and TET3 in bronchial cells, individually and in combination, and observed widespread DNA methylation by performing whole genome bisulfite sequencing. Overall design: Comparative DNA methylation analysis of WGBS data in HBEC3 defective cell (Control cells, RKO, YKO, TET2 KO, TET3KO, TET2/TET3 DKO, TET123 TKO and TET123/RYBP QKO )
Sample: HBEC3 cells, TET3KO2
SAMN29855122 • SRS14004834 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2500
Strategy: Bisulfite-Seq
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Construction protocol: Genomic DNA was extracted using a Quick-DNA Miniprep Plus kit (Zymo Research, D4070). The Accel-NGS Methyl-Seq DNA Library Kit (Swift Biosciences, 30024) was used according to the manufacturer's instructions to perform bisulfite conversion and library preparation. WGBS
Experiment attributes:
GEO Accession: GSM6364316
Links:
Runs: 1 run, 665.7M spots, 171.2G bases, 66.4Gb
Run# of Spots# of BasesSizePublished
SRR20334614665,726,325171.2G66.4Gb2023-07-10

ID:
23088582

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