GSM6364257: HBEC3 cells, Scr3, batch2; Homo sapiens; RNA-Seq - SRA

SRX16367646: GSM6364257: HBEC3 cells, Scr3, batch2; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 28.5M spots, 2.2G bases, 740.1Mb downloads

Submitted by: NCBI (GEO)

Study: Dysfunction of 5-methylcytosine oxidases and the Polycomb protein RYBP leads to widespread DNA hypermethylation and cell transformation [RNA-seq]

PRJNA860609 • SRP387317 • All experiments • All runs

show Abstracthide Abstract

To investigate the function of RYBP, YAF2, TET1, TET2, TET3 or the cooperative function in the human bronchial cells, we established defective clone cell lines in which each target gene has been knocked out by guide RNA We then performed gene expression profiling analysis from mRNA-seq. Overall design: Comparative gene expression profiling analysis of mRNA-seq data in HBEC3 defective cell (Control cells, RYBP KO, YAF2 KO, RYBP/YAF2 DKO , TET1/2/3 TKO and TET1/2/3/RYBP QKO ).

Sample: HBEC3 cells, Scr3, batch2

SAMN29855234 • SRS14004775 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNA was isolated with PureLink RNA Mini Kit (Invitrogen). Total RNA integrity was verified with an Agilent 2100 Bioanalyzer (Agilent Technologies) and quantified with a NanoDrop 8000 instrument (Thermo Fisher). RNA-seq libraries were prepared from total RNA with the KAPA RNA HyperPrep kit with RiboErase (KAPA Biosystems)

Experiment attributes:

GEO Accession: GSM6364257

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 28.5M spots, 2.2G bases, 740.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR20334555	28,471,112	2.2G	740.1Mb	2023-07-10

ID:: 23088523

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