GSM6340229: IonCode_0105; Homo sapiens; RNA-Seq - SRA

SRX16264739: GSM6340229: IonCode_0105; Homo sapiens; RNA-Seq
1 ION_TORRENT (Ion Torrent S5 XL) run: 9.9M spots, 1.1G bases, 579.5Mb downloads

External Id: GSM6340229_r1

Submitted by: The University of Chicago

Study: Effect of JAK-JAK Geometry on Gene Expression Levels in Cells Treated with Type I and III Interferons

PRJNA859134 • SRP386641 • All experiments • All runs

show Abstracthide Abstract

To evaluate the role of receptor geometry in interferon signaling, we engineered cell lines that express wild-type and mutant IFN? cognate receptors (IFN?R1) with rotated intracellular registers with respect to the associated JAK1. We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 different cell lines treated with 3 indicated interferons at 24hr. Overall design: Comparative gene expression profiling analysis of RNA-seq data for wild-type vs geometry-optimized HEK293 cells post-interferon treatment

Sample: IonCode_0105

SAMN29767446 • SRS13904595 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: GSM6340229

Instrument: Ion Torrent S5 XL

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was harvested using Monarch Total RNA miniprep kit (NEB). 1ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using Ion Torrent Ion 540 or 550 Library Prep Kit following manufacturer's protocols.

Runs: 1 run, 9.9M spots, 1.1G bases, 579.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR20230850	9,886,911	1.1G	579.5Mb	2024-07-01

ID:: 22974672

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