GSM6340068: UB cells,cKO,E16.5,biol rep 2 [B_2]; Mus musculus; RNA-Seq - SRA

SRX16252989: GSM6340068: UB cells,cKO,E16.5,biol rep 2 [B_2]; Mus musculus; RNA-Seq
1 ILLUMINA (HiSeq X Ten) run: 22.8M spots, 6.7G bases, 2Gb downloads

External Id: GSM6340068_r1

Submitted by: Shanghai Jiaotong University School of Medicine

Study: ASH2L controls ureteric bud morphogenesis via regulation of RET/GFRA1 signaling activity [RNA-seq]

PRJNA859015 • SRP386585 • All experiments • All runs

show Abstracthide Abstract

To elucidate the role of H3K4 methylation in metanephros development, we selectively inactivated ASH2L, core subunit of the COMPASS complex in mice UB lineage. We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 different FACS-sorted UB lineage cells at E16.5 of the control and cKO mouse embryos. Overall design: Comparative gene expression profiling analysis of RNA-seq data for FACS-sorted UB lineage cells at E16.5 of control and Ash2l cKO mouse embryos.

Sample: UB cells,cKO,E16.5,biol rep 2 [B_2]

SAMN29765766 • SRS13893028 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM6340068

Instrument: HiSeq X Ten

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA was harvested using mirVana miRNA Isolation Kit (Ambion). RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Then the libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions. The transcriptome sequencing and analysis were conducted by OE Biotech Co., Ltd. (Shanghai, China).

Runs: 1 run, 22.8M spots, 6.7G bases, 2Gb

Run	# of Spots	# of Bases	Size	Published
SRR20218823	22,785,511	6.7G	2Gb	2023-01-19

ID:: 22962730

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