GSM6311195: Control_2; Bos taurus; RNA-Seq - SRA

SRX16085555: GSM6311195: Control_2; Bos taurus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 37.3M spots, 7.5G bases, 2.2Gb downloads

External Id: GSM6311195_r1

Submitted by: Bioscience, Teagasc

Study: The transcriptomic response of bovine uterine tissue is altered in response to sperm from high and low fertility bulls

PRJNA856977 • SRP385529 • All experiments • All runs

show Abstracthide Abstract

The aim of this study was to profile the transcriptome and perform histological analysis of the bovine uterus in response to sperm from high fertility (HF) and low fertility (LF) bulls Overall design: Post-mortem uterine biopsies and uterine explants were collected from nulliparous heifers after a fixed time artificial insemination (AI) to a synchronized estrus with frozen-thawed semen from 5 HF and 5 LF bulls. Uterine biopsies were also collected from control (CTRL) heifers which were not inseminated. Differential gene expression in these tissues was assessed using RNA-Seq.

Sample: Control_2

SAMN29598311 • SRS13751462 • All experiments • All runs

Organism: Bos taurus

Library:

Name: GSM6311195

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Uterine samples were taken, flash frozen before subsequent RNA extraction in TRIzol reagent. Total RNA concentration was quantified using the Nanodrop ND-1000 UV-Vis Spectophotometer. Quality of RNA was ascertained with the use of 2100 Agilent Bioanalyzer . RNA libraries were prepared for sequencing using Illumina® TruSeq® Stranded mRNA Library preparation Kits

Runs: 1 run, 37.3M spots, 7.5G bases, 2.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR20047102	37,330,118	7.5G	2.2Gb	2022-08-01

ID:: 22773361

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