SRA

To investigate the functional role of EGFR in the formation of large vessels, we established a mesenchymal glioma stem cell line (GSC83) with intact EGFR and knocked down EGFR by Crispr-Cas9. We then performed single cells gene expression profiling analysis using data obtained from RNA-seq of 2 different tumours, derived by injection of GSC83 WT or GSC83 KO in NSG mice. In addition, GSC83 WT or GSC83 KO tumours in NSG mice were subjected to spatial gene expression profiling in situ using GeoMX platform. Overall design: Comparative single cells gene expression profiling analysis of RNA-seq data for 83 wild type EGFR and its EGFR knockdown derivative (GSC83 WT vs. GSC83 KO). For GeoMX spatial analysis, regions-of-interest were designated using anti-CD31 immunofluorescent tissue staining. Cells within/proximal to CD31-positive areas were profiled for mRNA expression.