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SRX15830668: GSM6261059: Shoot-Rep3-aoc-Salt-6h; Oryza sativa; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 41.6M spots, 12.5G bases, 3.6Gb downloads

External Id: GSM6261059_r1
Submitted by: IBMP, CNRS
Study: Jasmonate signaling controls negative and positive effectors of salt stress tolerance in rice
show Abstracthide Abstract
The JA deficient mutant (aoc) showed weaker symptoms than WT when both are exposed to salt. JAs signaling in WT, appeared then to impair salt tolerance and we were interested, through this transcriptomic approach, to highlight the JA-dependent component of the salt stress response that could explain the differential phenotype We report, for root and 2nd leaf, the compared transcriptomes of WT and aoc, before and at 3 different times (1 h, 6 h and 72 h) after salt exposure. The study reveals some key JA-regulated negative and positive effectors of salt stress tolerance in rice Overall design: WT and aoc seedlings were grown in hydroponic system for 11 days before being exposed to 100 mM NaCl solution. Samples were then harvested from root and 2nd leaf at two early (1 h and 6 h) and one late time point (72 h) to compare the transcriptionnal response of the two genotypes.
Sample: Shoot-Rep3-aoc-Salt-6h
SAMN29251928 • SRS13521950 • All experiments • All runs
Organism: Oryza sativa
Library:
Name: GSM6261059
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Root total RNA was extracted with Rneasy Plant Mini Kit (Quiagen) and 2nd leaf total RNA extracted with Tri-Reagent. After DNAse treatment, RNA was purified using NucleoSpin RNA Clean-up kit (Macherey-Nagel). After analysis with an Agilent 2 100 Bioanalyzer, all samples had a RIN value ≥ 6. illumina PE150 platform, performed by NOVOGENE (Cambridge, UK)
Runs: 1 run, 41.6M spots, 12.5G bases, 3.6Gb
Run# of Spots# of BasesSizePublished
SRR1978666541,640,62612.5G3.6Gb2023-06-20

ID:
22489113

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