GSM6135271: 003_hglPB3; Heterocephalus glaber; RNA-Seq - SRA

SRX15245112: GSM6135271: 003_hglPB3; Heterocephalus glaber; RNA-Seq
4 ILLUMINA (Illumina NovaSeq 6000) runs: 1.4G spots, 190.4G bases, 57.7Gb downloads

Submitted by: NCBI (GEO)

Study: Single-cell RNA-Sequencing maps the hematopoietic landscape in naked mole-rats [unfractionated PB]

PRJNA842730 • SRP384257 • All experiments • All runs

show Abstracthide Abstract

Naked mole-rats are a mammalian model organism of exceptional longevity. We mapped the hematopoietic hierarchy and used functional characterizations to define a purified stem and progenitor cell (HSPC) compartment similar to mouse LIN-/Sca-1+/Kit+ HSPCs or human LIN-/CD34+/CD38lo HSPCs. Overall design: Naked mole-rat peripheral blood was extracted, RBC-lysed and submitted to scRNA-Seq as unfractionated BM. The mRNA libraries were aligned to either the FRAMA bone marrow transcriptome assembly from our publication. Note: Raw data represents only gene expresion (GEX). Raw data for ADT not included.

Sample: 003_hglPB3

SAMN28209365 • SRS12978022 • All experiments • All runs

Organism: Heterocephalus glaber

Library:

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC SINGLE CELL

Selection: cDNA

Layout: PAIRED

Construction protocol: Cells were DAPI-sorted as viable unfractionated marrow tissue Chromium Single-Cell 3′ Library & Gel Bead Kit v3 (10X Genomics)

Experiment attributes:

GEO Accession: GSM6135271

Links:

NCBI link: NCBI Entrez (gds)

Runs: 4 runs, 1.4G spots, 190.4G bases, 57.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR19179865	347,868,852	48G	14.5Gb	2022-11-29
SRR19179866	352,709,902	48.7G	14.7Gb	2022-11-29
SRR19179867	337,435,802	46.6G	14.2Gb	2022-11-29
SRR19179868	341,562,247	47.1G	14.3Gb	2022-11-29

ID:: 21725651

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