GSM6128523: Tcrd.KO.BigTumor, S13; Mus musculus; RNA-Seq - SRA

SRX15226540: GSM6128523: Tcrd.KO.BigTumor, S13; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 64M spots, 10.3G bases, 4.7Gb downloads

External Id: GSM6128523_r1

Submitted by: Bioinformatics and Cancer Genomics, Olivia Newton John Cancer Research Institute

Study: Bulk RNA sequencing of epithelial cells from AOM-DSS mouse colorectal cancer (CRC)

PRJNA836957 • SRP374613 • All experiments • All runs

show Abstracthide Abstract

Gamma-delta T cells prevent CRC. To understand the criticial role of Gamma-delta T cells in CRC, AOM-DSS tumors from mice lacking TCR?d and with TCR?d cells were used to isolate epithelial cells to study gene expression by bulk RNA sequencing. Overall design: Bulk RNA sequencing of epithelial cells from mouse CRC tumors induced by AOM-DSS.

Sample: Tcrd.KO.BigTumor, S13

SAMN28180074 • SRS12960397 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM6128523

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Live Epcam+ epithelial cells were extracted from mouse tumors and RNA was isolated from epithelial cells for Nextseq sequencing using 150cyc High output kit (400M). Libraries were generated following the manufacturer's protocol.

Runs: 1 run, 64M spots, 10.3G bases, 4.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR19160130	63,977,623	10.3G	4.7Gb	2023-10-06

ID:: 21705046

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