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SRX15226257: GSM6128532: Tcf7WT-Tcrgd; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 162.1M spots, 19.3G bases, 7.2Gb downloads

External Id: GSM6128532_r1
Submitted by: Bioinformatics and Cancer Genomics, Olivia Newton John Cancer Research Institute
Study: Single cell gene expression analysis of mouse colon ?CRd+CD8a+ and TCRß+CD8a+ intraepitehlial lymphocytes (IELs)
PRJNA836955 • SRP374608 • All experiments • All runs
show Abstracthide Abstract
TCF-1 is highly expressed in colon T- IELs.T? understand the role of TCF-1 in regulating the gene expression of colon CD8+ T IELs, we isolated and sorted CD8a+TCRd+ and CD8a+TCRß+ from colons of E8?cre-Transgentic Tcf7 floxed mice or control Tcf7 floxed mice. Overall design: Single cell RNA sequencing of colon ?CR?d+CD8a+ and TCRß+CD8a+ IELs from mice with and without Tcf7 (E8ICre, Tcf7 floxed)
Sample: Tcf7WT-Tcrgd
SAMN28180068 • SRS12960119 • All experiments • All runs
Organism: Mus musculus
Library:
Name: GSM6128532
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: IELs were extracted from mice colons and sorted leukocytes were then loaded onto to the 10X ChromiumTM Single Cell 3' Chip to be encapsulated forming Gel Beads in Emulsion (GEM) and cDNA was generated as per 10X genomics protocol. Single cell RNA sequencing libraries were prepared according to 10X Genomics protocol and using the ChromiumTM Single Cell 3' Library and Gel Bead Kit (v2)
Runs: 1 run, 162.1M spots, 19.3G bases, 7.2Gb
Run# of Spots# of BasesSizePublished
SRR19159855162,070,91319.3G7.2Gb2023-10-06

ID:
21704763

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