GSM6124703: mutant 50 uM Hemin biol rep 2; Candida albicans; RNA-Seq - SRA

SRX15214068: GSM6124703: mutant 50 uM Hemin biol rep 2; Candida albicans; RNA-Seq
1 ILLUMINA (NextSeq 2000) run: 28.2M spots, 2.8G bases, 848.8Mb downloads

External Id: GSM6124703_r1

Submitted by: Kornitzer, Faculty of Medicine, Technion - IIT

Study: Effect of hemin and of HAP1 on C. albicans expression pattern

PRJNA836595 • SRP374410 • All experiments • All runs

show Abstracthide Abstract

To test the effect of hemin and of HAP1 in C. albicans, a hap1 mutant and it's reintegrant (wild-type) strain were grown in YPD to log phase, and then exposed or not to 50 microM hemin for 30'. Overall design: Comparative gene expression profiling analysis of RNA-seq data for hap1-/- cells with vector or with HAP1 reintegrant, with or without hemin, 3 biological replicates each (12 total).

Sample: mutant 50 uM Hemin biol rep 2

SAMN28164509 • SRS12951632 • All experiments • All runs

Organism: Candida albicans

Library:

Name: GSM6124703

Instrument: NextSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNA was extracted using hot phenol. NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, cat no. E7760, using 800ng total RNA as a starting material. mRNAs pull-down was performed using the Magnetic Isolation Module (NEB, cat no. E7490). After construction, the concentration of each library was measured using Qubit (Invitrogen) and the size was determined using the TapeStation 4200 with the High Sensitivity D1000 kit (cat no. 5067-5584).

Runs: 1 run, 28.2M spots, 2.8G bases, 848.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR19147320	28,212,235	2.8G	848.8Mb	2022-05-16

ID:: 21692392

SRA

Sequence Read Archive

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