GSM2029374: Het_RNA_Rep2; Homo sapiens; RNA-Seq - SRA

SRX1521202: GSM2029374: Het_RNA_Rep2; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 33.7M spots, 3.4G bases, 1.3Gb downloads

Submitted by: NCBI (GEO)

Study: Characterization of EZH2-deficient human embryonic stem cells [ChIP-seq and bulk RNA-seq]

PRJNA308197 • SRP068192 • All experiments • All runs

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Here we analyze histone modifications and transcriptome profiles of EZH2-deficient human embroynic stem cells Overall design: Histone modifications (H3K27me3, H3K4me3, H3K27ac and H3K4me1) and transcriptome (mRNA-Seq) from EZH2-/- (Null), Ezh2-/- +EZH2 (Null + EZH2), Ezh2 -/+ (Het) and EZH2 +/+ (WT) human ESC

Sample: Het_RNA_Rep2

SAMN04388045 • SRS1239404 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Human ESC were isolated by FACS straight into TRIzol LS (Life Technologies). Total RNA was purified and then cleaned up using an RNeasy kit (Qiagen). Indexed mRNA-Seq libraries were constructed from 500ng total RNA using the Tru-Seq RNA Library Prep Kit v2 (Illumina).

Experiment attributes:

GEO Accession: GSM2029374

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 33.7M spots, 3.4G bases, 1.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR3090634	33,710,971	3.4G	1.3Gb	2016-12-06

ID:: 2150992

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