GSM2027335: Host-P3_13_rep1; Pseudomonas aeruginosa; RNA-Seq - SRA

SRX1516057: GSM2027335: Host-P3_13_rep1; Pseudomonas aeruginosa; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 1.5M spots, 113.5M bases, 72.3Mb downloads

Submitted by: NCBI (GEO)

Study: Biological characterization of virulent therapeutic bacteriophage PAK_P3 infecting Pseudomonas aeruginosa.

PRJNA307603 • SRP068063 • All experiments • All runs

show Abstracthide Abstract

We have isolated and characterized several bacteriophages infecting Pseudomonas aeruginosa distantly related to Felix O1 virus and proposed they form a new subfamily named Felixounavirinae. The infectious cycle of bacteriophages belonging to this subfamily has not been studied yet in terms of gene expression. The present study reports the RNA-Seq analysis of bacteriophage PAK_P3 infecting PAK strain of P. aeruginosa. Overall design: RNA profile of Host and Phage at 0min, 3.5min and 13 min after infection of Pseudomonas aeruginosa PAK strain with the Pseudomonas phage PAK P3. Three biological replicates for each time point.

Sample: Host-P3_13_rep1

SAMN04383783 • SRS1234745 • All experiments • All runs

Organism: Pseudomonas aeruginosa PAK

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Cells were then lyzed in TRIzol,® total RNA was purified through a standard organic extraction and ethanol precipitation followed by DNase treatment (TURBO DNase®) and rRNA depletion (Ribo-Zero rRNA Removal Kit) RNA libraries were prepared for sequencing using standard Illumina protocols Truseq stranded RNA seq kit

Experiment attributes:

GEO Accession: GSM2027335

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 1.5M spots, 113.5M bases, 72.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR3084376	1,500,997	113.5M	72.3Mb	2016-07-25

ID:: 2144155

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity