GSM2027332: Host-P3_0_rep1; Pseudomonas aeruginosa; RNA-Seq - SRA

SRX1516053: GSM2027332: Host-P3_0_rep1; Pseudomonas aeruginosa; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 11.9M spots, 896.8M bases, 301.1Mb downloads

Submitted by: NCBI (GEO)

Study: Biological characterization of virulent therapeutic bacteriophage PAK_P3 infecting Pseudomonas aeruginosa.

PRJNA307603 • SRP068063 • All experiments • All runs

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We have isolated and characterized several bacteriophages infecting Pseudomonas aeruginosa distantly related to Felix O1 virus and proposed they form a new subfamily named Felixounavirinae. The infectious cycle of bacteriophages belonging to this subfamily has not been studied yet in terms of gene expression. The present study reports the RNA-Seq analysis of bacteriophage PAK_P3 infecting PAK strain of P. aeruginosa. Overall design: RNA profile of Host and Phage at 0min, 3.5min and 13 min after infection of Pseudomonas aeruginosa PAK strain with the Pseudomonas phage PAK P3. Three biological replicates for each time point.

Sample: Host-P3_0_rep1

SAMN04383809 • SRS1234749 • All experiments • All runs

Organism: Pseudomonas aeruginosa PAK

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Cells were then lyzed in TRIzol,® total RNA was purified through a standard organic extraction and ethanol precipitation followed by DNase treatment (TURBO DNase®) and rRNA depletion (Ribo-Zero rRNA Removal Kit) RNA libraries were prepared for sequencing using standard Illumina protocols Truseq stranded RNA seq kit

Experiment attributes:

GEO Accession: GSM2027332

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 11.9M spots, 896.8M bases, 301.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR3084373	11,887,550	896.8M	301.1Mb	2016-07-25

ID:: 2144151

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