Name: GSM6070920
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: The olfactory epithelium was dissected out from the nasal cavity for each sample. The total RNA was isolated by TRIzol Reagent and subsequently purified with the RNeasy Mini Kit (Qiagen, Hilden, Germany). The quantity and purity of the isolated RNA were measured by NanoDrop ND-1000 Spectrophotometer before cDNA library construction. The cDNA libearies were constructed by TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA). Briefly, mRNA was purified from total RNA (1 μg) by oligo(dT)-coupled magnetic beads and fragmented into small pieces under elevated temperature. The first-strand cDNA was synthesized using reverse transcriptase and random primers. After the generation of double-strand cDNA and adenylation on 3' ends of DNA fragments, the adaptors were ligated, and the samples were purified with AMPure XP system (Beckman Coulter, Beverly, USA). The quality of the libraries was assessed on the Agilent Bioanalyzer 2100 system and a Real-Time PCR system.