GSM6065200: Hep3B, DNMT1-S878A, 25mM Glucose, Thiamet-G, Replicate 1;... - SRA

SRX14992344: GSM6065200: Hep3B, DNMT1-S878A, 25mM Glucose, Thiamet-G, Replicate 1; Homo sapiens; Bisulfite-Seq
1 OXFORD_NANOPORE (PromethION) run: 8M spots, 42.5G bases, 34.2Gb downloads

External Id: GSM6065200_r1

Submitted by: City of Hope

Study: Inhibition of DNMT1 methyltransferase activity via glucose-regulated O-GlcNAcylation alters the epigenome

PRJNA831845 • SRP372162 • All experiments • All runs

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Profiling of DNA methylation Overall design: Comparative DNA methylation profiling analysis of Oxford nanopore PromethION data for 5mM or 25mM glucose treated Hep3B cells and its mutants (myc-DNMT1-WT, myc-DNMT1-S878A).

Sample: Hep3B, DNMT1-S878A, 25mM Glucose, Thiamet-G, Replicate 1

SAMN27765601 • SRS12741678 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: GSM6065200

Instrument: PromethION

Strategy: Bisulfite-Seq

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Construction protocol: Genomic DNA was extracted using DNA mini kit with G-tip (Qiagen). 2 ug of genomic DNA was used for the construction of sequencing libraries. DNA library for nanopore sequencing were constructed using Genomic DNA Ligation Kit following manufacturer's protocols.

Runs: 1 run, 8M spots, 42.5G bases, 34.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR18914403	8,025,657	42.5G	34.2Gb	2023-07-22

ID:: 21447075

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