GSM6046589: 3-1-KO1; Mus musculus; RNA-Seq - SRA

SRX14870958: GSM6046589: 3-1-KO1; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 28.4M spots, 5.7G bases, 1Gb downloads

External Id: GSM6046589_r1

Submitted by: DePinho Lab, Genomic Medicine, University of Texas, MD Anderson Cancer Center

Study: Synthetic Essentiality of Tryptophan 2,3-dioxygenase 2 in APC-Mutated Colorectal Cancer II

PRJNA827171 • SRP370519 • All experiments • All runs

show Abstracthide Abstract

Comparative transcriptomic analysis of TAMs isolated from APC-WT and APC-KO MC38 orthotopic tumors express higher levels of multiple classical M2-like markers (CD163, CCL22, YM1) compared to APC-WT tumors after re-normalized the read counts of multiple M2 macrophage markers with housekeeping gene read counts (GAPDH and ACTB). Overall design: Transcriptomic analysis of macrophages isolated from APC-WT and APC-KO MC38 orthotopic tumors.

Sample: 3-1-KO1

SAMN27603920 • SRS12624976 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM6046589

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Stained cells were sorted as CD45+, CD11b+, and F4/80+. Total RNA was isolated from sorted cells and were sequenced by Ultra low Input RAN sequencing (Illumina NexteraXT) with six replicates of each sample.

Runs: 1 run, 28.4M spots, 5.7G bases, 1Gb

Run	# of Spots	# of Bases	Size	Published
SRR18771583	28,373,305	5.7G	1Gb	2022-08-03

ID:: 21301508

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