GSM6034964: Skin of a patient affected by diffuse (anti Scl70/RNA Pol... - SRA

SRX14787060: GSM6034964: Skin of a patient affected by diffuse (anti Scl70/RNA Pol III) sclerodermia [C_24_D]; human skin metagenome; OTHER
1 ILLUMINA (Illumina MiSeq) run: 200 spots, 109,958 bases, 153,956b downloads

External Id: GSM6034964_r1

Submitted by: Experimental and Clinical Biomedical Sciences, University of Florence

Study: 16S V3-V4 -based microbial profiling of stool, skin and saliva samples of patients affected by systemic sclerosis (SSc)

PRJNA824808 • SRP368271 • All experiments • All runs

show Abstracthide Abstract

Systemic sclerosis (SSc) is a complex and still unclear rare disease associated with fibrosis in multiple organs. Microbiota has recently emerged as an important environmental factor in SSc pathogenesis, either at gut, oral and skin level. Overall design: DNA extraction from stool, skin and saliva samples collected from 26 patients affected by SSc, generatoin of amplicons of the variable V3–V4 region of the bacterial 16S rRNA gene and paired-end sequencing (2?×?300 cycles) on the Illumina MiSeq platform.

Sample: Skin of a patient affected by diffuse (anti Scl70/RNA Pol III) sclerodermia [C_24_D]

SAMN27478833 • SRS12545953 • All experiments • All runs

Organism: human skin metagenome

Library:

Name: GSM6034964

Instrument: Illumina MiSeq

Strategy: OTHER

Source: GENOMIC

Selection: other

Layout: PAIRED

Construction protocol: Genomic DNA extraction with DNeasy PowerLyzer PowerSoil Kit (Qiagen, Hilden, Germany) Illumina 16S Metagenomic Sequencing Library Preparation protocol ( see https://emea.support.illumina.com)

Runs: 1 run, 200 spots, 109,958 bases, 153,956b

Run	# of Spots	# of Bases	Size	Published
SRR18685982	200	109,958	153,956b	2023-12-28

ID:: 21169964

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