GSM5953334: Endoderm_St10_rep3; Xenopus laevis; RNA-Seq - SRA

SRX14462560: GSM5953334: Endoderm_St10_rep3; Xenopus laevis; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 21.4M spots, 1.6G bases, 656.6Mb downloads

External Id: GSM5953334_r1

Submitted by: Molecular Biosciences, Northwestern University

Study: Transcriptome sequencing of Xenopus laevis animal caps at six time points in transit from pluripotency to 4 lineages: epidermal, neural, ventral mesoderm and endoderm

PRJNA816095 • SRP364006 • All experiments • All runs

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The purpose of this study is to examine transciptome dynamics at high resolution to 4 separate lineages. Here we provide snapshots of the Xenopus laevis transcriptome at Nieuwkoop and Faber stages 9., 10, 10.5, 11, 12 and 13 for the epidmeral, neural, ventral mesoderm and endoderm lineage Overall design: Methods: mRNA profiles of Xenops animal cap populations at st9, 10, 10.5, 11, 12 13 in transit to the following lineages: epidermal (untreated), neural (100ng/uL noggin), ventral mesoderm (20ng/uL BMP4/7), endoderm (160ng/uL activin) were generated by Next Gen Sequencing

Sample: Endoderm_St10_rep3

SAMN26658565 • SRS12266107 • All experiments • All runs

Organism: Xenopus laevis

Library:

Name: GSM5953334

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Animal caps were flash frozen in trizol. RNA was harvested using Trizol reagent extraction from Xenopus animal caps. 1ug total RNA was used for construction of sequencing libraries Illumina TruSeq mRNA-Seq Library Prep Kit- standard protocols

Runs: 1 run, 21.4M spots, 1.6G bases, 656.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR18325086	21,403,748	1.6G	656.6Mb	2022-10-23

ID:: 20636086

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